3C-like Proteinase Rabbit Polyclonal Antibody

CAT#: TA373100

SARS-CoV-2 3CLpro Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody (TA373100) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CLpro fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 3CLpro Rabbit pAb (TA373100) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: SARS-CoV-2
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of SARS-CoV-2 3CLpro.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 43740578 SARS-CoV-2
  UniProt ID P0DTC2
• Background: [Replicase polyprotein 1ab]: Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.