EEA1 Rabbit Polyclonal Antibody

CAT#: TA372450

EEA1 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-4: NIH/3T3 RAW264.7 and 293T cell Human metastatic malignant melanoma tissue lysates Primary antibody: TA372450 (EEA1 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 500-2000
  WB positive control: NIH/3T3, RAW264.7 and 293T cell, Human metastatic malignant melanoma tissue lysates
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human EEA1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 163 kDa
• Gene Name: early endosome antigen 1
• Database Link:
  Entrez Gene 8411 Human
  UniProt ID Q15075
• Background: EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking. The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes. The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin. Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion.
• Synonyms: MST105; MSTP105; ZFYVE2