E2 230K (UBE2O) Rabbit Polyclonal Antibody

CAT#: TA371000

UBE2O rabbit polyclonal antibody

Product Images

Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA371000 (UBE2O Antibody) at dilution 1/120 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA371000 (UBE2O Antibody) at dilution 1/120, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA371000 (UBE2O Antibody) at dilution 1/120 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA371000 (UBE2O Antibody) at dilution 1/120, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: IHC: 100-300
  Positive control: Human thyroid cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human UBE2O
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Gene Name: ubiquitin conjugating enzyme E2 O
• Database Link:
  Entrez Gene 63893 Human
  UniProt ID Q9C0C9
• Background: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
• Synonyms: E2-230K; FLJ12878; KIAA1734