ECH1 Rabbit Polyclonal Antibody

CAT#: TA370562

ECH1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-5: Human fetal liver tissue Hela 293T HepG2 and A549 cell lysates Primary antibody: TA370562 (ECH1 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 7 seconds

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA370562 (ECH1 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA370562 (ECH1 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human fetal liver tissue, Hela, 293T, HepG2 and A549 cell lysates
  IHC: 50-300
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ECH1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 36 kDa
• Gene Name: enoyl-CoA hydratase 1, peroxisomal
• Database Link:
  Entrez Gene 1891 Human
  UniProt ID Q13011
• Background: This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
• Synonyms: HPXEL