HLA-DRB4 Rabbit Polyclonal Antibody

CAT#: TA370348

HLA-DRB4 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Raji cell lysate Primary antibody: TA370348 (HLA-DRB4 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA370348 (HLA-DRB4 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA370348 (HLA-DRB4 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Raji cell lysate
  IHC: 20-100
  Positive control: Human tonsil
  Predicted cell location: Cell membrane
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human HLA-DRB4
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 30 kDa
• Gene Name: major histocompatibility complex, class II, DR beta 4
• Database Link:
  Entrez Gene 3126 Human
  UniProt ID P13762
• Background: HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Synonyms: DR-4; DR4; DRB4; HLA-DR4B