TAZ (WWTR1) Rabbit Polyclonal Antibody

CAT#: TA368080

WWTR1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: HEPG2 Jurkat A431 and NIH/3T3 cell lysates Primary antibody: TA368080 (WWTR1 Antibody) at dilution 1/650 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 200-1000
  WB positive control: HEPG2，Jurkat，A431 and NIH/3T3 cell lysates
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human WWTR1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 44 kDa
• Gene Name: WW domain containing transcription regulator 1
• Database Link:
  Entrez Gene 25937 Human
  UniProt ID Q9GZV5
• Background: The transcriptional co-activator with PDZ-binding motif (TAZ) is a 14-3-3-binding molecule. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. TAZ may link events at the plasma membrane and cytosketeton to nuclear transcription in a manner that can be regulated by 14-3-3. TAZ shares homology with the WW domain of Yes-associated protein (YAP) and functions as a transcriptional co-activator by binding to the PPXY motif present on transcription factors. TAZ recognizes immunoreactive protein bands in lysates from MDCK, NIH-3T3 and 293T cells. In addition, COS7, Hep G2, CHO and HeLa cells express endogenous TAZ. 14-3-3 binding requires TAZ phosphorylation on a single Serine 89 residue, resulting in the inhibition of TAZ transcriptional co-activation through 14-3-3-mediated nuclear export.
• Synonyms: DKFZp586I1419; FLJ27004; FLJ45718; TAZ