C13orf8 (CHAMP1) Rabbit Polyclonal Antibody

CAT#: TA367899

CHAMP1 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: A172 cell lysate Primary antibody: TA367899 (CHAMP1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA367899 (CHAMP1 Antibody) at dilution 1/35 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA367899 (CHAMP1 Antibody) at dilution 1/35, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA367899 (CHAMP1 Antibody) at dilution 1/35 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA367899 (CHAMP1 Antibody) at dilution 1/35, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: A172 cell lysate
  IHC: 25-100
  Positive control: Human ovarian cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human CHAMP1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 89 kDa
• Gene Name: chromosome alignment maintaining phosphoprotein 1
• Database Link:
  Entrez Gene 283489 Human
  UniProt ID Q96JM3
• Background: CHAMP1 (chromosome alignment maintaining phosphoprotein 1), also known as ZNF828 or C13orf8, is a 812 amino acid protein containing one C2H2-type zinc finger. CHAMP1 is required for the proper alignment of chromosomes during metaphase, undergoing CDK1-dependent phosphorylation at multiple sites during mitosis. The phosphorylation counteracts the negative chromosomal alignment regulation of the zinc-finger domain of CHAMP1. One region of CHAMP1, the FPE region, is responsible for spindle and kinetochore localization, which is essential for proper chromosome alignment. CHAMP1 interacts with MAD2L2, PGOZ, CBX1, CBX3 and CBX5, and may recruit CENPE and CENPF to the kinetochore. The CHAMP1 gene is located on chromosome 13 and is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat and chicken.
• Synonyms: RGD1311283; Zfp828