Endothelin 1 (EDN1) Rabbit Polyclonal Antibody

CAT#: TA364182

Rabbit polyclonal anti Endothelin-1 (hu, bo, ca, ms, po, rt); neat antiserum, Unspecific Crossreactivity Profile

Product Images

Typical titration curve of Endothelin-1 in a competitive ELISA with this antibody

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Endothelin-1. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Human, Bovine, Dog, Mouse, Porcine, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys- Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-OH, (Disulfide bonds between Cys1 and Cys15/Cys3 and Cys11) coupled to carrier protein.
• Formulation: Neat undiluted antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 50µl distilled water. This will give the equivalent of undiluted antiserum.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: endothelin 1
• Database Link:
  Entrez Gene 1906 Human
  UniProt ID P05305
• Background: Endothelin (ET-1) is a 21 amino acid peptide, also known as preproendothelin-1 (PPET1), that is produced by the vascular endothelium from a 39 amino acid precursor, big ET-1, through the actions of an endothelin converting enzyme (ECE) found on the endothelial cell membrane. ET-1 is involved in regulation of cardiovascular functions where it has been implicated in the pathogenesis of hypertension, coronary vasospasm, and heart failure. It is also known to causes fibrosis of the vascular cells as well as to stimulate production of reactive oxygen species. This antibody was generated by immunization of rabbits with Endothelin-1 coupled to a carrier protein.
• Synonyms: ET1; HDLCQ7; PPET1; Preproendothelin-1