Cd163 Mouse Monoclonal Antibody [Clone ID: ED2]

CAT#: TA363848

CD163 (scavenger receptor, haemoglobin-haptoglobin receptor), mouse anti rat, clone ED2

Product Images

TA363848, Clone ED2, rat spleen, frozen section

Specifications

Product Data

• Clone Name: ED2
• Applications: IHC
• Recommended Dilution: Tested for immunohistochemistry (IHC); has been described to work in FACS, immunprecipitation. Approximate working dilution for IHC: Frozen sections: 1µg/ml (1:1000) Paraffin sections: 20µg/ml (1:50 Proteinase K pretreatment for antigen retrieval is recommended Optimal dilutions should be determined by the end user. Suggested positive control: Rat spleen.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat spleen cells.
• Specificity: Rat: macrophages. Other species: not tested
  
  Epitope: The antigen has been described as CD163, epitope not further characterized.
  
  Distribution: Isolated cells: Monocytes and dendritic cells harvested from spleen and lymph nodes, and granulocytes harvested from the peritoneal cavity are negative with ED 2.
• Formulation: Affinity purified, liquid. Supplied as a 0.25ml solution. This stock solution contains 1mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), no stabilizer and 0.09% sodium azide as a preservative.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
• Gene Name: CD163 molecule
• Database Link:
  Entrez Gene 312701 Rat
  UniProt ID D3Z9U2
• Background: Monoclonal antibody ED2 reacts with a membrane antigen (175, 160 and 95kDa) on resident rat macrophages. Monocytes, dendritic cells (spleen and lymph node), peritoneal granulocytes and other cell types are negative. ED2 discriminates between thymic cortical (positive for ED2) and medullary (negative for ED2) macrophages. It is useful to stain synovial lining cells and Kupffer cells in the liver. The antigen has recently been shown to be identical with CD163 (Polfliet, M.M.J. et al [2002], PhD Thesis, Vrije Universiteit, Amsterdam).
• Synonyms: M130; MM130