Ionotropic Glutamate receptor 2 (GRIA2) Rabbit Polyclonal Antibody

CAT#: TA363813

GRIA2 Antibody - N-terminal region

Product Images

WB Suggested Anti-GRIA2 Antibody Titration: 2.5ug/ml ELISA Titer: 1:1562500 Positive Control: HepG2 cell lysate

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  Primary Antibodies Brain slices were incubated overnight in a solution of PBST (0.2%) + 10% Normal GOAT serum + a monoclonal mouse anti-Parvalbumin antibody (1:2000)+ rabbit anti-GRIA2 (Aviva systems Biology, 1ug/mL)and gently shaken overnight at 4 C. Secondary Antibodies Samples were washed 6x in PBS (10 min each at RT) and exposed to the following secondary antibodies o/n at 4 C: PBST (0.3%) + 10% normal goat serum + anti-mouse Alexa 488 (1:1000) + Anti-rabbit Alexa 555 (1:1000) for two hours. Samples were washed 6x in PBST mounted. Images acquired with a confocal microscope at 63X.

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  Application: Immunohistochemistry Species+tissue/cell type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop How many ug'sof tissue/cell lysate run on the gel: 11 m Mouse Gut Tissue Primary antibody dilution: 1:50 Secondary antibody: Goat anti-rabbit-cy3 Secondary antibody dilution: 1:1500

Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GRIA2
• Specificity: Expected reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
  Homology: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 99kDa
• Gene Name: glutamate ionotropic receptor AMPA type subunit 2
• Database Link:
  NP_000817
  Entrez Gene 14800 MouseEntrez Gene 2891 Human
  UniProt ID P42262
• Background: GRIA2 is one of the glutamate receptors, which are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with mul
• Synonyms: GluA2; gluR-B; GluR-K2; GLUR2; GluR2; GLURB; HBGR2

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction