UBE2M Rabbit Polyclonal Antibody

CAT#: TA356299

UBE2M Antibody - N-terminal region

Product Images

WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml Positive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B

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  Sample Type:2. mouse brain extracts (80ug) Primary Antibody Dilution:Â 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution:Â 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceÂ

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  Host: Rabbit Target Name: UBE2M Sample Type: Human MCF7 Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in MCF7

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  Host: Rabbit Target Name: UBE2M Sample Type: Human 721_B Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in 721_B

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  Sample Type:Â NT2 cells Red:Â Antibody Blue:Â DAPI Primary Dilution:Â 1ug/50ul antibody Secondary Antibody:Â Alexa goat anti-rabbit 594 Image Submitted by:Â Yuzhi Chen, University of Arkansas for Medical Sciences Â

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  Sample Type: Human brain stem cells Primary Antibody Dilution: 1:500 Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution: 1:1000 Color/Signal Descriptions: UBE2M: Red DAPI:Blue Gene Name: UBE2M Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

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Specifications

Product Data

• Applications: IHC, WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21kDa
• Gene Name: ubiquitin conjugating enzyme E2 M
• Database Link:
  NP_003960
  Entrez Gene 22192 MouseEntrez Gene 9040 Human
  UniProt ID P61081
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
• Synonyms: hUbc12; UBC-RS2; UBC12

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis