CDK4 Rabbit Polyclonal Antibody

CAT#: TA355441

CDK4 Antibody - C-terminal region

Product Images

WB Suggested Anti-CDK4 Antibody Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell

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  Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: CDK4 Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Friedrich-schiller University Jena CDK4 is supported by BioGPS gene expression data to be expressed in HCT116

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  Lanes: Lane1: 30 ug untreated human HCT116 cell lysate Lane2-3: 30 ug genotoxic agent treated human HCT116 cell lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: CDK4 Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena CDK4 is supported by BioGPS gene expression data to be expressed in HCT116

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  Host: Rabbit Target Name: CDK4 Sample Tissue: Human A549 Whole Cell Antibody Dilution: 1ug/ml

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  Rabbit Anti-CDK4 antibody Catalog Number: TA355441 Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X

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  CDK4 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with TA355441 with 1:200 dilution. Western blot was performed using TA355441 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: CDK4 IP with TA355441 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.

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Specifications

Product Data

• Applications: IHC, IP, WB
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Pig, Rabbit, Rat, Sheep
  Homology: Cow: 75%; Dog: 83%; Guinea Pig: 83%; Horse: 83%; Human: 100%; Pig: 92%; Rabbit: 83%; Rat: 83%; Sheep: 75%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 34kDa
• Gene Name: cyclin-dependent kinase 4
• Database Link:
  NP_000066
  Entrez Gene 1019 Human
  UniProt ID P11802
• Background: Cdk4 is probably involved in the control of the cell cycle.
• Synonyms: CMM3; MGC14458; PSK-J3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer, T cell receptor signaling pathway, Tight junction