B7-1 (CD80) Rabbit Polyclonal Antibody

CAT#: TA355032

Rabbit Polyclonal Anti-CD80 Antibody

Product Images

Western blot analysis of CD80 in EL4 cell lysate with CD80 antibody at 1ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 2ug/ml.

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  Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1-2µg/mL.IHC: 2µg/mL. Immunoflorescence start at 20µg/mL.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: CD80 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human CD80. The immunogen is located within amino acids 60 - 110 of CD80.
• Formulation: CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: CD80 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 32 kDa; Observed: 50 kDa
• Gene Name: CD80 molecule
• Database Link:
  NP_005182
  Entrez Gene 941 Human
  UniProt ID P33681
• Background: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
• Synonyms: B7; BB1; CD28LG; CD28LG1; LAB7
• Note: CD80 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL.