Lymphocyte Activation Gene 3 (LAG3) Rabbit Polyclonal Antibody

CAT#: TA355030

Rabbit Polyclonal Anti-LAG3 Antibody

Product Images

Western blot analysis of LAG3 in mouse liver tissue lysate with LAG3 antibody at 1ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of LAG3 in human tonsil tissue with LAG3 antibody at 5ug/ml.

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  Immunofluorescence of LAG3 in human tonsil tissue with LAG3 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1-2µg/mL. IHC: 5µg/mL.Immunoflorescence start at 20µg/mL.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3. The immunogen is located within amino acids 110 - 160 of LAG3.
• Formulation: LAG3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: LAG3 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 55 kDa; Observed: 54 kDa
• Gene Name: lymphocyte activating 3
• Database Link:
  NP_002277
  Entrez Gene 3902 Human
  UniProt ID P18627
• Background: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).
• Synonyms: CD223; FDC; LAG-3
• Note: LAG3 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.