SOX9 Rabbit Polyclonal Antibody

CAT#: TA354842

Rabbit anti SOX-9 (paired S181) Polyclonal Antibody

Product Images

WB: The whole cell lysate derived from LNCaP prostate Carcinoma were immunoblotted Rabbit anti-SOX-9 (Paired 181) at 1:500 (Lane 1). Lane 2 is a negative control

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  DB: 1 ug peptide was blot onto NC membrane A: SOX-9 (pS181) B: SOX-9 (Paired S181) (Nonphospho); C: Non-related phosphospecific PP were blotted at a 1:1000 dilution by: 1: Rabbit anti-SOX-9 (pS181); 2: Rabbit anti-SOX-9 (paired 181),

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding the epitope –RKSVK- without phosphorylation. This sequence is identical among human, rat, mouse, bovine, chicken, and dog.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56 kDa
• Gene Name: SRY-box 9
• Database Link:
  NP_000337
  Entrez Gene 20682 MouseEntrez Gene 140586 RatEntrez Gene 6662 Human
  UniProt ID P48436
• Background: The SOX9 is a member of the SOX [Sry-related high-mobility group (HMG) box] family of HMG DNA-binding domain transcription factors and is required for the development and differentiation of multiple cell lineages. SOX9 is expressed in primary prostate cancers in vivo, at a higher frequency in recurrent prostate cancer and in prostate cancer cell lines (LNCaP, CWR22, PC3, and DU145). It has been reported that the cAMP/PKA stimulation induces SOX-9 nuclear translocation via facilitating its nuclear import by the phosphorylation of SOX-9 at Ser64 and Ser181. SOX9 in prostate basal cells supports the development and maintenance of the luminal epithelium and that a subset of prostate cancer cells may escape basal cell requirements through SOX9 expression.
• Synonyms: CMD1; CMPD1; SRA1; SRXX2; SRXY10

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors