ROCK2 Rabbit Polyclonal Antibody

CAT#: TA354745

Rabbit anti Rho Kinase/ROCKII (pT249) Polyclonal Antibody

Product Images

WB: The cell lysate derived from Conditioned 3T3 cell was immunoprecipitated by Rabbit anti-Rho Kinase (pT249), then immunoprobed by the same antibody at 1:500 (lane 1). Lane 2 is a negative control.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat, Chicken, Bovine, Canis
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the epitope -CDTAV- with a single phosphorylation site Thr249 of human Rho Kinase/RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 165 kDa
• Gene Name: Rho associated coiled-coil containing protein kinase 2
• Database Link:
  NP_001308572
  Entrez Gene 19878 MouseEntrez Gene 25537 RatEntrez Gene 9475 Human
  UniProt ID O75116
• Background: Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
• Synonyms: ROCK-II

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway