BCAS2 Rabbit Polyclonal Antibody

CAT#: TA349703

Rabbit Polyclonal Anti-BCAS2 Antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: A172 cells 231 cells Primary antibody: TA349703 (BCAS2 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA349703 (BCAS2 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA349703 (BCAS2 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349703 (BCAS2 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349703 (BCAS2 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: A172 and 231 cells
  IHC: 50-200
  Positive control: Human colon cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human BCAS2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26 kDa
• Gene Name: breast carcinoma amplified sequence 2
• Database Link:
  NP_005863
  Entrez Gene 68183 MouseEntrez Gene 10286 Human
  UniProt ID O75934
• Background: BCAS2 (breast carcinoma amplified sequence 2), also designated DAM1 (DNA amplified in mammary carcinoma 1 protein) or spliceosome-associated SPF 27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. BCAS2 is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TR∫ (thyroid hormone receptor beta), ERå (estrogen receptor alpha), ER∫, PR (progesterone receptor), and PPAR© (Peroxisome proliferator-activated receptor gamma). BCAS2 functions as an ER coactivator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.
• Synonyms: DAM1; Snt309; SPF27

Reference Data

• Protein Pathways: Spliceosome