Isocitrate dehydrogenase (IDH1) Rabbit Polyclonal Antibody

CAT#: TA349598

Rabbit Polyclonal Anti-IDH1 Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: 231 cells hela cells Primary antibody: TA349598 (IDH1 Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA349598 (IDH1 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA349598 (IDH1 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349598 (IDH1 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349598 (IDH1 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: 231 and hela cells
  IHC: 50-200
  Positive control: Human liver cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human IDH1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47 kDa
• Gene Name: isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• Database Link:
  NP_005887
  Entrez Gene 15926 MouseEntrez Gene 24479 RatEntrez Gene 3417 Human
  UniProt ID O75874
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence.
• Synonyms: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways