SESN2 Rabbit Polyclonal Antibody

CAT#: TA349117

Rabbit Polyclonal Anti-SESTRIN2 Antibody

Product Images

Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 5 ug/mL.

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  Immunofluorescence of SESTRIN2 in mouse kidney tissue with SESTRIN2 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: SESTRIN2 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 54 kDa; Observed: 53 kDa
• Gene Name: sestrin 2
• Database Link:
  NP_113647
  Entrez Gene 83667 Human
  UniProt ID P58004
• Background: SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).
• Synonyms: HEL-205; MRD2; ZIR8