PI 3 Kinase regulatory subunit 4 (PIK3R4) Rabbit Polyclonal Antibody

CAT#: TA349094

Rabbit Polyclonal Anti-PIK3R4 Antibody

Product Images

Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 ug/mL.

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  Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: PIK3R4 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 149 kDa; Observed: 140 kDa
• Gene Name: phosphoinositide-3-kinase regulatory subunit 4
• Database Link:
  NP_055417
  Entrez Gene 30849 Human
  UniProt ID Q99570
• Background: PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).
• Synonyms: SCCD; TERE1