SMURF 2 (SMURF2) Rabbit Polyclonal Antibody

CAT#: TA349045

Rabbit Polyclonal Anti-SMURF2 Antibody

Product Images

Western blot analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1ug/ml.

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  Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2ug/ml.

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  Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2ug/ml.

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  Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5ug/ml.

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  Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20ug/ml.

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  Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20ug/ml.

• Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20ug/ml.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2. The immunogen is located within amino acids 140 - 190 of SMURF2.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: SMURF2 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 82 kDa; Observed: 85 kDa
• Gene Name: SMAD specific E3 ubiquitin protein ligase 2
• Database Link:
  NP_073576
  Entrez Gene 64750 Human
  UniProt ID Q9HAU4
• Background: SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).
• Synonyms: VUR3