hSET1 (SETD1A) Rabbit Polyclonal Antibody

CAT#: TA347284

Rabbit Polyclonal Setd1a Antibody

Product Images

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Setd1a, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 186 kDa) is indicated on the right.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Setd1a. The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2, 500.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100) ; Western blotting (1:500)
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-Setd1a antibody: mouse Setd1a (Set domain containing 1A), using three KLH-conjugated synthetic peptides: two containing an amino acid sequence from the N-terminal part of the protein and one containing an amino acid sequence from th
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SET domain containing 1A
• Database Link:
  NP_055527
  Entrez Gene 233904 Mouse
  UniProt ID O15047
• Background: SETD1A (UniProt/Swiss-Prot entry O15047) is a component of the SET1 histone methyltransferase (HMT) complex. This complex specifically methylates lysine 4 of histone H3, but only if the neighboring lysine 9 residue is not yet methylated. Methylation of H3K4 represents a specific tag for epigenetic transcriptional activation. SETD1A shows a non-overlapping localization with SETD1B, suggesting that both proteins play a specific role in the epigenetic control of chromatin structure and gene expression.
• Synonyms: KMT2F; Set1; Set1A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation