Retinoic Acid Receptor alpha (RARA) Rabbit Polyclonal Antibody

CAT#: TA347271

Rabbit Polyclonal RARA Antibody

Product Images

WB using the antibody against RARA, diluted 1:750 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

• 

  Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human RARA. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:2, 400.

• 

  ChIP assays were performed using NB4 cells, the antibody against RARA and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. Image shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.

• 

  a) ChIP-seq results obtained with the antibody against RARA ChIP was performed as described above and the IP'd DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ChIP/ChIP-seq (4 µl/ChIP); ELISA (1:50); Western blotting (1:750)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-RARA antibody: human RARA (Retinoic Acid Receptor alpha) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: retinoic acid receptor alpha
• Database Link:
  NP_000955
  Entrez Gene 5914 Human
  UniProt ID P10276
• Background: RARA (UniProtKB/Swiss-Prot entry P10276) is a receptor for retinoic acid, a vitamin A metabolite, which directly regulates gene expression in target cells by binding to specific DNA response elements. In the absence of its ligand, this receptor represses transcription through the recruitment of specific corepressors and of HDAC's, whereas binding of retinioc acid causes the recruitment of coactivators and HAT's. Translocations involving the RARA gene, often leading to a RARA/PML fusion protein, are a major cause of acute promyelocytic leukemia.
• Synonyms: NR1B1; RAR

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer