SMAD5 Mouse Monoclonal Antibody [Clone ID: 4B10-B10-B6]

CAT#: TA347048

Mouse Monoclonal SMAD5 (C-terminus) Antibody

Specifications

Product Data

• Clone Name: 4B10-B10-B6
• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:75, FC: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The immunogen for SMAD5 antibody: purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60 kDa
• Gene Name: SMAD family member 5
• Database Link:
  NP_001001420
  Entrez Gene 4090 Human
  UniProt ID Q99717
• Background: SMAD5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. It is a receptor-regulated SMAD (R-SMAD), and acts as an intracellular signal transducer for the transforming growth factor beta superfamily. SMAD5 is activated through serine phosphorylation by BMP (bone morphogenetic proteins) type 1 receptor kinase. It is cytoplasmic in the absence of its ligand and migrates into the nucleus upon phosphorylation and complex formation with SMAD4. Here the SMAD5/SMAD4 complex stimulates the transcription of target genes.
• Synonyms: DWFC; JV5-1; MADH5

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway