alpha Tubulin (TUBA4A) Rabbit Polyclonal Antibody

CAT#: TA345670

Rabbit Polyclonal Anti-TUBA4A Antibody

Product Images

Sample Type: 1. Human NT-2 cells (60ug); 2. and 3. mouse brain extracts (80ug); 4. rat brain extract (80ug); Primary Antibody Dilution: 2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: 1: 20, 000;

• 

  WB Suggested Anti-TUBA4A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Placenta

• 

  Sample Type: Mouse brain stem cells Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody: ilution: 1: 1000; Color/Signal Descriptions: TUBA4A: Red DAPI: Blue; Gene Name: TUBA4A

• 

  Amount and Sample Type: 500 ug mouse brain homogenate; Amount of IP Antibody: 6 ug; Primary Antibody: TUBA4A; Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution: 1: 5000; Gene Name: TUBA4A;

• 

  User supplied image. Ana C. for TA345670 on 06/02/2023: IF staining of MDA-MB-231cells. F-actin staining in red, nuclei in blue and alpha-tubulin in red. Antibody Dilution: 1: 500 Secondary Antibody: Goat anti-rabbit Alexa-Fluor 488 Secondary Antibody: dilution: 1: 1000. F-actin staining with Alexa Fluor 594 Phalloidin 1:40 Color/Signal Descriptions: TUBA4A: green F-actin: red DAPI: Blue Gene Name: TUBA4A

Show all 5 images

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: WB, IHC, IP
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-TUBA4A antibody: synthetic peptide directed towards the middle region of human TUBA4A. Synthetic peptide located within the following region: YAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: tubulin alpha 4a
• Database Link:
  NP_005991
  Entrez Gene 22145 MouseEntrez Gene 7277 Human
  UniProt ID P68366
• Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Synonyms: ALS22; H2-ALPHA; TUBA1
• Note: Immunogen Sequence Homology: Dog: 100%; Pig: 100%; Rat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Sheep: 100%; Bovine: 100%; Rabbit: 100%; Guinea pig: 100%

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection