AIM2 Mouse Monoclonal Antibody [Clone ID: 10M2B3]

CAT#: TA337163

Mouse Monoclonal AIM2 Antibody (10M2B3)

Product Images

Western Blot: AIM2 Antibody (10M2B3) TA337163 - analysis using AIM2 antibody. Recombinant human AIM2 probed with AIM2 antibody at 2 ug/ml.

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  Western Blot: AIM2 Antibody (10M2B3) TA337163 - Western blot analysis of Daudi lysate (35 ug per lane, RIPA buffer) using AIM2 antibody (TA337163) at 0.3ug/ml. Band observed at ~38kDa. (Expected MW of 39.0kDa according to NP_004824.1)

Specifications

Product Data

• Clone Name: 10M2B3
• Applications: WB
• Recommended Dilution: Western Blot: 0.3-2ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised against recombinant human AIM2 protein
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: absent in melanoma 2
• Database Link:
  NP_004824
  Entrez Gene 9447 Human
  UniProt ID O14862
• Background: AIM2 (Absent in Melanoma-2) is an interferon-inducible cytosolic protein of the IFI20X/IFI16 family, which is essential for an effective innate immune response. It plays a key role in the defence against bacterial and viral DNA. AIM2 is essential for inflammasome activation in response to Francisella tularensis, vaccinia virus and Listeria monocytogenes (Fernandes-Alnemri T et al, 2009). It recognizes cytosolic dsDNA and forms a Caspase-1 activating inflammasome with ASC (apoptosis-associated speck-like protein containing a CARD) protein (Jones JW et al, 2010). The C-terminal HIN domain binds double stranded DNA and acts as a cytosolic dsDNA sensor. The pyrin domain allows for the association with ASC and formation of the inflammasome. Formation of the AIM2 inflammsome leads to the processing and release of mature IL-1Beta and IL-18 and host cell death (Hornung V et al, 2009). AIM2 plays a role in tumorigenic reversion and may control cell proliferation. It may repress NF-kappaB transcriptional activity.AIM2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM2 induces the expression of invasion-associated genes such as VIM and MCAM, whereas downregulates ANXA10 and CDH1. AIM2 reduces breast cancer proliferation and mammary tumor growth and shows a high frequency of frameshift mutations in microsatellite unstable (MSI-H) gastric, endometrial and colorectal cancers.Defects in AIM2expression within immune cells contribute to increased susceptibility to lupus. AIM2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients. It may serve as a potential therapeutic gene for future development of AIM2-based gene therapy for human breast cancer (Liu G et al, 2004).
• Synonyms: PYHIN4

Reference Data

• Protein Pathways: Cytosolic DNA-sensing pathway