TLR7 Rabbit Polyclonal Antibody

CAT#: TA337080

Rabbit Polyclonal TLR7 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: TLR7 Antibody TA337080 - Analysis using the Azide Free version of TA337080. Staining of Raw 246.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.

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  Immunohistochemistry-Paraffin: TLR7 Antibody - BSA Free TA337080 - Analysis of a FFPE tissue section of human skin using 1:200 dilution of TLR7 antibody (TA337080). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

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  Flow Cytometry: TLR7 Antibody TA337080 - An intracellular stain was performed on THP-1 cells with TA337080 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

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  Western Blot: TLR7 Antibody TA337080 - Analysis using the Azide Free version of TA337080. Detection of TLR7 in RAW cell lysate using this antibody.

Specifications

Product Data

• Applications: Dot, FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:10-1:2000, Flow (Intracellular), Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry: 1:200, Immunoprecipitation, Dot Blot, Proximity Ligation Assay, Bioactivity, Immunohistochemistry-Frozen: 1:10-1:2000, Flow Cytometry: 2-5 ug/ 1x10^6 cells, Western Blot: 1-3 ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 706-749 of human TLR7; GenBank no. gb|AAF78035.1|AF245702_1. It will cross-react with mouse TLR7. In human Ramos cells, additional bands are seen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 7
• Database Link:
  NP_057646
  Entrez Gene 170743 MouseEntrez Gene 317468 RatEntrez Gene 51284 Human
  UniProt ID Q9NYK1
• Synonyms: TLR7-like

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway