Raptor (RPTOR) Rabbit Polyclonal Antibody

CAT#: TA337071

Rabbit Polyclonal RPTOR Antibody

Product Images

Immunocytochemistry/Immunofluorescence: RPTOR Antibody TA337071 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RPTOR at a 1:100 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: RPTOR Antibody TA337071 - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.

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  Immunohistochemistry-Paraffin: RPTOR Antibody TA337071 - Analysis of human small intestine using Raptor antibody at 1:100.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 1: 1000-1:2000, Immunohistochemistry-Paraffin: 1: 100, Immunocytochemistry/ Immunofluorescence: 1:100
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 900-950 of human Raptor was used as the immunogen for this antibody.
• Formulation: Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: regulatory associated protein of MTOR complex 1
• Database Link:
  NP_001156506
  Entrez Gene 74370 MouseEntrez Gene 57521 Human
  UniProt ID Q8N122
• Synonyms: KOG1; Mip1

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway