TLR5 Mouse Monoclonal Antibody [Clone ID: 19D759.2]

CAT#: TA337067

Mouse Monoclonal TLR5 Antibody (19D759.2)

Product Images

Flow Cytometry: TLR5 Antibody (19D759.2) TA337067 - Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of TLR5 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR5 antibody. this antibody secondary antibody was used in this test.

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  Flow Cytometry: TLR5 Antibody (19D759.2) TA337067 - Analysis using the PE conjugate of TA337067. Staining of CD14-/CD83+/TLR5+ diffferentiated myeloid dendritic cells (mDCs). PBMCs were stimulated 5 days with GM-CSF+IL4 prior to the staining. The cells were stained for surface markers CD14 and CD83 FITC, and TLR5 PE. CD14- cells were gated and stained with PE-conjugated isotype control (mouse IgG2a), and FITC-conjugated CD83 (left). CD14- cells were gated and stained with 1 ug PE-conjugated TLR5 , and FITC-conjugated CD83 (right).

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  Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) TA337067 - Analysis of TLR5 in FFPE mouse brain tissue using TLR5 antibody at 5 ug/ml.

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  Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) TA337067 - Human skin stained with TLR5 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

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  Immunohistochemistry-Paraffin: TLR5 Antibody (19D759.2) TA337067 - Analysis of FFPE tissue section of normal human skin using 5 ug/ml concentration of TLR5 antibody (clone 19D759.2). Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification]

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Specifications

Product Data

• Clone Name: 19D759.2
• Applications: Dot, FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 1-3 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Immunohistochemistry-Frozen: 10 ug/ml, Flow Cytometry: 0.5-1 ug/10^6 cells, Flow (Cell Surface), Flow (Intracellular): 0.5-1 ug/10^6 cells, Immunohistochemistry: 1:10-1:500, Dot Blot, Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Immunoprecipitation
• Reactivities: Human, Mouse, Canine
• Host: Mouse
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 5
• Database Link:
  NP_003259
  Entrez Gene 53791 MouseEntrez Gene 7100 Human
  UniProt ID O60602
• Synonyms: MELIOS; SLE1; SLEB1; TIL3
• Note: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway