HIPK3 Rabbit Polyclonal Antibody

CAT#: TA336992

Rabbit Polyclonal HIPK3 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: HIPK3 Antibody TA336992 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIPK3 TA336992 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: HIPK3 Antibody TA336992 - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Immunohistochemistry-Paraffin: HIPK3 Antibody TA336992 - Analysis of human testis using this antibody at 10 ug/ml.

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  Immunohistochemistry-Paraffin: HIPK3 Antibody TA336992 - Analysis of HIPK3 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunocytochemistry/ Immunofluorescence: 1:200, Western Blot: 0.5-2 ug/ml, Immunohistochemistry-Paraffin: 5-10 ug/ml
• Reactivities: Human, Mouse, Rat, Canine, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 850-900 of human HIPK3 was used as the immunogen.
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: homeodomain interacting protein kinase 3
• Database Link:
  NP_005725
  Entrez Gene 15259 MouseEntrez Gene 83617 RatEntrez Gene 10114 Human
  UniProt ID Q9H422
• Synonyms: DYRK6; FIST3; PKY; YAK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors