GLI2 Rabbit Polyclonal Antibody

CAT#: TA336950

Rabbit Polyclonal GLI-2 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: GLI-2 Antibody TA336950 - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.

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  Immunocytochemistry/Immunofluorescence: GLI-2 Antibody TA336950 - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (TA336950) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Immunohistochemistry: GLI-2 Antibody TA336950 - Analysis of GLI-2 in mouse heart.

Specifications

Product Data

• Applications: ICC/IF, IHC
• Recommended Dilution: Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin: 1:200 - 1:300, Immunohistochemistry: 1:200 - 1:300, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:200
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human Gli2 protein (between residues 300-400) [UniProt P10070]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: GLI family zinc finger 2
• Database Link:
  NP_005261
  Entrez Gene 14633 MouseEntrez Gene 2736 Human
  UniProt ID P10070
• Background: Gli2, also known as THP, is a transcription factor which binds DNA through zinc finger motifs. Belonging to the C2H2-Zinc finger type subclass of the Gli family, Gli2 is a mediator of Shh (sonic hedgehog) signaling, a pathway that is aberrantly activated in many types of cancers. Specifically, it has been found that the anti-apoptotic protein BCL-2 is up regulated by Gli2, leading to carcinogenesis. The protein encoded by the Gli2 gene localizes to the cytoplasm and activates patched (Drosophila homolog) gene expression, where it has been thought to play an important role during embryogenesis. Mutations in the Gli2 gene are found in various syndromes, including Pallister-Hall Syndrome, Greig cephalopolysyndactyly syndrome, and preaxial polydactyl type IV. Recently, the interaction between the hedgehog signaling pathway and Gli2 has been implicated in medullary thyroid carcinoma (PMID: 23410206).
• Synonyms: CJS; HPE9; PHS2; THP1; THP2
• Note: In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer