ABCG5 Mouse Monoclonal Antibody [Clone ID: 1B5E10]

CAT#: TA336947

Mouse Monoclonal ABCG5 Antibody (1B5E10)

Product Images

Flow Cytometry: ABCG5 Antibody (1B5E10) TA336947 - Flow cytometry analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).

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  Immunohistochemistry: ABCG5 Antibody (1B5E10) TA336947 - IHC analysis of paraffin-embedded human cervical cancer cancer tissues with DAB staining.

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  Immunohistochemistry: ABCG5 Antibody (1B5E10) TA336947 - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.

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  Western Blot: ABCG5 Antibody (1B5E10) TA336947 - Western blot analysis of ABCG5 in (1) HL7702, (2) RAJI and (3) Jurkat cell lysate.

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  Western Blot: ABCG5 Antibody (1B5E10) TA336947 - Western blot analysis of ABCG5 in human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)

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  Western Blot: ABCG5 Antibody (1B5E10) TA336947 - Western blot analysis of ABCG5 in (1) HEK293 and (2) ABCG5 hIgGFc transfected HEK293 cell lysate.

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Specifications

Product Data

• Clone Name: 1B5E10
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 1:200 - 1:400, ELISA: 1:10000, Immunohistochemistry: 1:200 - 1:2000, Western Blot: 1:500 - 1:2000, Immunohistochemistry-Paraffin: 1:200 - 1:2000, ICC/IF (Negative)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Partial recombinant human ABCG5 (between residues 300-500) expressed in E. coli [UniProt Q9H222].
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATP binding cassette subfamily G member 5
• Database Link:
  NP_071881
  Entrez Gene 64240 Human
  UniProt ID Q9H222
• Background: ABCG5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCG5 is a member of the White subfamily. It functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. ABCG5 is expressed in a tissue-specific manner in the liver, colon, and intestine. Mutations in this protein may contribute to sterol accumulation and atheroschlerosis, and they have been observed in patients with sitosterolemia (1).
• Synonyms: STSL

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters