NFAT2 (NFATC1) Rabbit Polyclonal Antibody

CAT#: TA336469

Rabbit Polyclonal NFATC1/NFAT2 Antibody

Product Images

Simple Western: NFATC1/NFAT2 Antibody TA336469 - Lane view shows a specific band for NFAT2 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody TA336469 - NFAT2 antibody was tested in Raw 246.7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.

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  Western Blot: NFATC1/NFAT2 Antibody TA336469 - Analysis of human (A)Ramos and (B) mouse RAW lysate using NFATc1 antibody at 1 ug/ml.

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  Western Blot: NFATC1/NFAT2 Antibody TA336469 - Analysis of NFATc1 in Ramos cell lysate. (1) Without blocking peptide. (2) With blocking peptide.

Specifications

Product Data

• Applications: ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Simple Western: 1:100, Immunocytochemistry/ Immunofluorescence: 1:20, Western Blot: 1-3 ug/ml, Immunohistochemistry
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nuclear factor of activated T-cells 1
• Database Link:
  NP_765976
  Entrez Gene 18018 MouseEntrez Gene 4772 Human
  UniProt ID O95644
• Background: NFAT (nuclear factor of activated T cell) transcription factors play critical roles in gene transcription during immune responses. The NFAT complex is made up of NFATc (NFATc1 - 4) and AP-1 transcription factor. NFAT proteins are regulated by Ca2+. NFATc1 is a substrate protein phosphatase calcineurin, a critical mediator of calcium signals during Tcell activation. NFATc1 activation requires the translocation of the transcription factor into the nucleus, a process that is mediated by calcineurin.
• Synonyms: NF-ATC; NF-ATc1.2; NFAT2; NFATc
• Note: Immunoreative bands can be blocked by preincubation of NB100-56732 with immunizing peptide. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon testing material.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway