DAP3 Mouse Monoclonal Antibody [Clone ID: 42C617.1.2]

CAT#: TA336396

Mouse Monoclonal DAP3 Antibody (42C617.1.2)

Product Images

Western Blot: DAP3 Antibody (42C617.1.2) TA336396 - Analysis of DAP-3 in A) human brain, B) Jurkat and C) NIH 3T3 lysate using this antibody at 5 ug/ml.

Specifications

Product Data

• Clone Name: 42C617.1.2
• Applications: WB
• Recommended Dilution: Western Blot: 3-5 ug/ml
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: The antibody was raised against a synthetic peptide corresponding to amino acids 51-66 of human DAP-3 protein.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: death associated protein 3
• Database Link:
  NP_387506
  Entrez Gene 65111 MouseEntrez Gene 7818 Human
  UniProt ID P51398
• Background: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.
• Synonyms: bMRP-10; DAP-3; MRP-S29; MRPS29

Reference Data

• Protein Families: Druggable Genome