RANKL (TNFSF11) Mouse Monoclonal Antibody [Clone ID: 12A668]

CAT#: TA336377

Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody (12A668)

Product Images

MQDL detects EMT biomarkers in clinical bone tissue specimens.A representative specimen of human prostate cancer bone metastasis co-expressed high levels of epithelial EpCAM, and mesenchymal RANKL and vimentin proteins. ×400.

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  Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) TA336377 - Analysis using Azide Free version of TA336377. FFPE human liver stained with TRANCE/TNFSF11/RANK L antibody, peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.

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  Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) TA336377 - Analysis using Azide Free version of TA336377. Human lymph node lysate (35ug per lane, RIPA buffer). Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)

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  Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) TA336377 - TRANCE was detected in immersion fixed mouse splenocytes using anti-human/mouse/rat mouse monoclonal antibody (Catalog # TA336377) for 1 hour at room temperature. Cells were stained using NL557 (red) fluorescent anti-mouse secondary antibodies (Catalog # NL007) and counterstained with DAPI (blue).

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  Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) TA336377 - Analysis using Azide Free version of TA336377. FFPE human lymph node probed with TRANCE/TNFSF11/RANK L antibody.

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  Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) TA336377 - Analysis of transfected cell lysate was probed with TRANCE/TNFSF11/RANK L antibody.

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Specifications

Product Data

• Clone Name: 12A668
• Applications: ChIP, ELISA, FC, IA, ICC/IF, IHC, WB
• Recommended Dilution: ELISA, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Flow (Cell Surface), Immunoassay, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 5 ug/ml, Immunocytochemistry/ Immunofluorescence: 20 ug/ml, Western Blot: 0.5-2 ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen (NP_003692).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35 kDa
• Gene Name: tumor necrosis factor superfamily member 11
• Database Link:
  NP_003692
  Entrez Gene 21943 MouseEntrez Gene 117516 RatEntrez Gene 8600 Human
  UniProt ID O14788
• Background: TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
• Synonyms: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TRANCE
• Note: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction