Ryanodine Receptor 3 (RYR3) Rabbit Polyclonal Antibody

CAT#: TA329026

Rabbit polyclonal Anti-Ryanodine Receptor 3

Product Images

Western blot analysis of rat brain membranes: 1. Anti-Ryanodine Receptor 3 antibody, (1:200). 2. Anti-Ryanodine Receptor 3 antibody, preincubated with the control peptide antigen.

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  Expression of Ryanodine Receptor 3 in rat cortex. Immunohistochemical staining of rat brain frozen sections with Anti-Ryanodine Receptor 3 antibody, (red), (1:100). Ryanodine Receptor 3 is widely expressed in neurons of the cortex. Hoechst 33342 is used as the counterstain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human Ryanodine Receptor 3. Intracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Reconstitution Method: Add 50 ul double distilled water (DDW) to the lyophilized powder.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ryanodine receptor 3
• Database Link:
  NP_001027
  Entrez Gene 6263 Human
  UniProt ID Q15413
• Background: It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation. The primary intracellular Ca2+ storage/release organelles in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells. The ER and SR contain two Ca2+ release channel families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs). The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3). The Ryanodine receptors are homotetrameric proteins generating a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine (poisonous alkaloid found in the South American plant Ryania speciosa to the Ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel. RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain.
• Synonyms: RYR-3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway