WDHD1 Mouse Monoclonal Antibody [Clone ID: 20G10]

CAT#: TA328108

Mouse Monoclonal anti-AND-1 (WDHD1) Antibody

Product Images

Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.

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  Formalin-fixed paraffin-embedded human kidney tissue was stained with 20G10 at 15 ug/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.

Specifications

Product Data

• Clone Name: 20G10
• Applications: IHC, WB
• Recommended Dilution: WB, IHC
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: Recombinant (partial), C-terminal
• Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 126 kD
• Gene Name: WD repeat and HMG-box DNA binding protein 1
• Database Link:
  NP_009017
  Entrez Gene 218973 MouseEntrez Gene 11169 Human
  UniProt ID O75717
• Background: The 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting.
• Synonyms: AND-1; AND1; CHTF4; CTF4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors