H3FT (HIST3H3) Mouse Monoclonal Antibody [Clone ID: 11D8]

CAT#: TA328045

Mouse Monoclonal anti-Histone H3 Phospho (Ser10) Antibody

Product Images

293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells).

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  Exponentially growing Hela cells were stained with purified phospor-Histone H3 (Ser10), clone 11D8 antibody, followed by Alexa Fluor® 488 conjugated to anti-mouse IgG and DAPI. The phosphor-Histone staining is specific on metaphase cells only.

Specifications

Product Data

• Clone Name: 11D8
• Applications: IF, WB
• Recommended Dilution: WB, IF
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Modified synthetic peptide conjugated to KLH
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
  Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN₃.
• Concentration: lot specific
• Purification: The antibody was purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: histone cluster 3, H3
• Database Link:
  NP_003484
  Entrez Gene 8290 Human
  UniProt ID Q16695
• Background: Histone H3 is phosphorylated at serine 10 during mitosis and found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
• Synonyms: g; H3; H3.4; H3FT; H3t

Reference Data

• Protein Pathways: Systemic lupus erythematosus