LAT Mouse Monoclonal Antibody [Clone ID: 11B.12]

CAT#: TA327925

Mouse Monoclonal anti-LAT Antibody

Product Images

Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12.

Specifications

Product Data

• Clone Name: 11B.12
• Applications: WB
• Recommended Dilution: WB, IP
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: GST fusion protein corresponding to residues 31-233 of human LAT, cytoplasmic domain
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kD but expected at 36-38 kD
• Gene Name: linker for activation of T-cells
• Database Link:
  NP_001014987
  Entrez Gene 16797 MouseEntrez Gene 27040 Human
  UniProt ID O43561
• Background: LAT is a transmembrane molecule that mediates signaling following the engagement of a number of cell surface receptors including TCR during mature T cell activation, pre-TCR during T cell development, Fc RIII on NK cells, and FcεRI on mast cells.LAT is multiply phosphorylated on tyrosines by ZAP-70 and Syk, which leads to downstream effects that include release of intracellular calcium, PKC activation, MAPK activation and cytoskeletal reorganization.Palmitoylation of LAT is required for its localization to lipid rafts and efficient phosphorylation.
• Synonyms: LAT1; pp36

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway