CD8A Mouse Monoclonal Antibody [Clone ID: C8/144B]

CAT#: TA327655

Mouse Monoclonal CD8 Antibody

Product Images

Immunohistochemistry staining of Paraffin Tonsil tissue by CD8 antibody (dilution: 1:25 - 1:100; visualization of staining: Membranous)

Specifications

Product Data

• Clone Name: C8/144B
• Applications: IHC
• Recommended Dilution: IHC: 1:25 - 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Formulation: This antibody is supplied as cell culture supernatant diluted in tris buffered saline, pH 7.3-7.7, with 1% BSA and <0.1% sodium azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD8a molecule
• Database Link:
  NP_741969
  Entrez Gene 925 Human
  UniProt ID P01732
• Synonyms: CD8; Leu2; MAL; p32
• Note: The CD8 (cluster of differentiation 8) antigen is a cell surface glycoprotein found on most cytotoxic T-lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). TCR is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T-helper and cytotoxic T-cells that can be distinguished by their expression of CD4 and CD8 respectively. CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. A primary function of CD8 is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. The CD8 co-receptor is predominantly expressed on the surface of suppressor and cytotoxic T-cells at a low level by NK cells, large granular lymphocyte leukemia, and some T-ALL/T-LBL.For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4, is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas (CD4+/CD8-). Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway