Grm5 Mouse Monoclonal Antibody [Clone ID: S75-33]

CAT#: TA326541

Mouse Monoclonal Anti-mGluR1/5 Antibody

Product Images

Western blot analysis of mGluR1-5 in rat brain membrane tissues, using a 1:1000 dilution of the antibody

Specifications

Product Data

• Clone Name: S75-33
• Applications: WB
• Recommended Dilution: WB: 1ug/ml, IHC: 0.1-1ug/ml, IF: 1-10ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b
• Formulation: PBS pH7.4, 50% glycerol
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glutamate metabotropic receptor 5
• Database Link:
  NP_058708
  Entrez Gene 2915 HumanEntrez Gene 108071 MouseEntrez Gene 24418 Rat
  UniProt ID P31424
• Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient acrossthe plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient . Theyare present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge,others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, thepassage is governed by a gate which is controlled by chemical or electrical signals, temperature, ormechanical forces.There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated,other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of thereceptor protein. The other gated classifications include activation and inactivation by second messengers, inward rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels .The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fastsynaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers .
• Synonyms: GPRC1E; mGlu5; MGLUR5; OTTHUMP00000165953; OTTHUMP00000165957
• Note: Detects ~130kDa (both mGluR1 and mGluR5)