CENPA Mouse Monoclonal Antibody [Clone ID: 5A7-2E11]

CAT#: TA326348

Mouse monoclonal CENP-A Antibody

Product Images

Western blot analysis of CENP-A in U2OS lysates using a 1:1000 dilution of the antibody

Specifications

Product Data

• Clone Name: 5A7-2E11
• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A.
• Formulation: PBS, 50% glycerol
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: centromere protein A
• Database Link:
  NP_001800
  Entrez Gene 1058 Human
  UniProt ID P49450
• Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain.CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specializedcentromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
• Synonyms: CenH3; CENP-A
• Note: Detects ~18kDa. Recognizes Human CENP-A