MDM2 Rabbit Polyclonal Antibody

CAT#: TA325681

Rabbit Polyclonal MDM2 (Ser166) Antibody (Phospho-specific)

Product Images

Western blot analysis on COS7 cell lysate using Phospho-MDM2 (Ser166) Antibody

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  Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue using Phospho- MDM2 (Ser166) Antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200; IF 1:100-1:500
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against a synthesized A synthesized peptide derived from human MDM2 around the phosphorylation site of Sersine 166.
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MDM2 proto-oncogene
• Database Link:
  NP_001138809
  Entrez Gene 17246 MouseEntrez Gene 4193 Human
  UniProt ID Q00987
• Background: This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
• Synonyms: ACTFS; hdm2; HDMX

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis