EIF4EBP1 Rabbit Polyclonal Antibody

CAT#: TA325450

Rabbit Polyclonal 4E-BP1 (Thr36) Antibody (Phospho-specific)

Product Images

Western blot analysis of 4E-BP1 phosphorylation expression in EGF treated MDA-MB-435 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Threonine 36
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  NP_004086
  Entrez Gene 13685 MouseEntrez Gene 116636 RatEntrez Gene 1978 Human
  UniProt ID Q13541
• Background: 4E-BP1 binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation.
• Synonyms: 4E-BP1; 4EBP1; BP-1; PHAS-I

Reference Data

• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway