DDIT3 Rabbit Polyclonal Antibody

CAT#: TA324451

Rabbit polyclonal DDIT3 Antibody (C-term A135)

Product Images

DDIT3 Antibody (C-term A135) (Cat. #TA324451) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).

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  IF image of Hela cell stained with DDIT3 Antibody (C-term A135) (Cat#TA324451/SA101207AA).Hela cells were incubated with DDIT3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). DDIT3 immunoreactivity is localized to Cytoplasm significantly.

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  DDIT3 Antibody (C-term A135) (Cat. #TA324451) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivities: Mouse (Predicted: Bovine, Hamster)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19175 Da
• Gene Name: DNA damage inducible transcript 3
• Database Link:
  NP_001181982
  Entrez Gene 13198 Mouse
  UniProt ID P35638
• Synonyms: CEBPZ; CHOP; CHOP-10; CHOP10; GADD153

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway