alpha 1 Fetoprotein (AFP) Rabbit Polyclonal Antibody

CAT#: TA321282

Anti-AFP Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: 231 Hela and HepG2 cell lysates Primary antibody: TA321282 (AFP Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA321282 (AFP Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA321282 (AFP Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: 231, Hela and HepG2 cell lysates
  IHC: 30-150
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 350 amino acids of Human Alpha-fetoprotein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: alpha fetoprotein
• Database Link:
  NP_001125
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: This gene encodes alpha-fetoprotein; a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However; hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin; and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms; and binds copper; nickel; fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
• Synonyms: AFPD; FETA; HPAFP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein