AKT1 Mouse Monoclonal Antibody [Clone ID: 18F3.H11]

CAT#: TA319561

Mouse monoclonal Akt phospho T308 antibody

Product Images

Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band (s): none

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  ELISA of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Antigen: Unconjugated Akt phospho T308 and AKT non-phospho T308. Coating amount: 0.1 ug per well. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 5 ug/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Akt phospho T308 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)

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  Immunohistochemistry of Mouse Anti-AKT phospho T308 Biotin Conjugated at 20X (left) and 40X (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker, Heat, EDTA pH 9.5 Pressure Cooker Primary antibody: 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Specifications

Product Data

• Clone Name: 18F3.H11
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1:20,000, WB: 1:500 - 1:3,000, IHC: 20 ug/ml
• Reactivities: Human, Mouse, Rat, Monkey
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  NP_001014431
  Entrez Gene 11651 MouseEntrez Gene 24185 RatEntrez Gene 697747 MonkeyEntrez Gene 207 Human
  UniProt ID P31749
• Synonyms: AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
• Note: Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway