Bach2 Rabbit Polyclonal Antibody

CAT#: TA319354

Rabbit polyclonal anti-Bach2 antibody

Product Images

Western Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 ug per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 91.7 kDa, ~130 kDa for Bach2. Other band (s): none.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:5000, WB: 1 ug/mL, IP: 1 ug/mL
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BTB and CNC homology, basic leucine zipper transcription factor 2
• Database Link:
  NP_031547
  Entrez Gene 12014 Mouse
  UniProt ID P97303
• Synonyms: OTTHUMP00000016868
• Note: During an immune response to infectious diseases and cancer, B-cells undergo activation in response to antigen, and differentiate either into short-lived plasma cells, whose numbers contract following withdrawal of the antigenic stimulus, or into memory B-cells. The formation of B-cell memory is the major goal of vaccination, since memory B-cells are able to produce antibodies specific to infections and cancer that can last for the lifetime of the host. Recently it has been shown that the formation of long-lived memory B-cells is dependent upon the transcription factor Bach2, since knockout mice lacking the gene fail to generate class-switched IgG antibody responses and all B-cells undergo default plasma cell differentiation upon antigenic stimulation. While the mechanism by which Bach2 controls B-cell memory fate has been elucidated (it binds and represses the promoter of the gene that encodes the plasma cell master regulatory transcription factor Blimp-1), the upstream mechanism by which the function of Bach2 is regulated is unknown. There is evidence suggesting that Bach2 is phosphorylated in B-cells following stimulation and suspect that this modification may allow the B-cell signaling apparatus to control Bach2 activity and therefore memory fate decisions. Anti-Bach2 is ideal for researchers interested in Cancer and DNA Damage & Repair research.