hHR23A (RAD23A) Goat Polyclonal Antibody

CAT#: TA319249

Goat polyclonal anti-HR23A antibody

Product Images

WB showing Anti-HR23A antibody detects endogenous human HR23A. Reactivity against HeLa nuclear extract (lane 1) and HeLa (lane 2), A431 (lane 3), Jurkat (lane 4) and 293 whole cell lysates (lane 5). Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using HRP conjugated Rb-a-Goat IgG and chemilumi-nescence reagent with a 30-min exposure time.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:2,000
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 122-135 of human HR23A protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAD23 homolog A, nucleotide excision repair protein
• Database Link:
  NP_001257291
  Entrez Gene 5886 Human
  UniProt ID P54725
• Synonyms: HHR23A; HR23A
• Note: HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair