Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

CAT#: TA319229

Rabbit polyclonal Cyclin B1 antibody

Product Images

WB analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG.

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  WB analysis using anti-cyclin B antibody shows detection of human Cyclin B present in asynchronous HN30 cell lysates.HN30 cells are from head and neck cancer tumors that over express cyclin B and D. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody.Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG.

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  anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000, IP: 1:100
• Reactivities: Human, Rat, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
• Formulation: None
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin B1
• Database Link:
  NP_114172
  Entrez Gene 25203 RatEntrez Gene 268697 MouseEntrez Gene 891 Human
  UniProt ID P14635
• Synonyms: CCNB
• Note: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation